OpenMS
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FeatureLinkerUnlabeled

Groups corresponding features from multiple maps.

potential predecessor tools → FeatureLinkerUnlabeled → potential successor tools
FeatureFinderCentroided
(or another feature detection algorithm)
ProteinQuantifier
MapAlignerPoseClustering
(or another map alignment algorithm)
TextExporter
SeedListGenerator

This tool provides an algorithm for grouping corresponding features in multiple runs of label-free experiments. For more details and algorithm-specific parameters (set in the INI file) see "Detailed Description" in the algorithm documentation or the INI file table below.

FeatureLinkerUnlabeled takes several feature maps (featureXML files) and stores the corresponding features in a consensus map (consensusXML file). Feature maps can be created from MS experiments (peak data) using one of the FeatureFinder TOPP tools.

Advanced users can convert the consensusXML generated by this tool to EDTA using FileConverter and plot the distribution of distances in RT (or m/z) between different input files (can be done in Excel). The distribution should be Gaussian-like with very few points beyond the tails. Points far away from the Gaussian indicate a too wide tolerance. A Gaussian with its left/right tail trimmed indicates a too narrow tolerance.

See also
FeatureLinkerUnlabeledQT FeatureLinkerLabeled

The command line parameters of this tool are:

INI file documentation of this tool: